SILICON-STABILISED HYBRID LIPID NANOPARTICLE · RESEARCH USE ONLY
One reagent, from in vitro to in vivo
See what your payload does, not what the reagent did. Deliver in vivo without reformulating. For siRNA, mRNA and other nucleic acids.
Launching soon. Evaluation kits go to registered labs first.
Better payload decisions with Bio‑Courier® TL
Conventional transfection reagents often compromise cell viability, introducing biological noise. Bio‑Courier® TL keeps cells viable, so what the assay measures is the payload. And because the same chemistry carries that payload into animal studies, it can be evaluated in vivo without the confounding effects introduced by a different carrier chemistry.
Expression data that reflect the payload
Conventional transfection reagents
Cell toxicity creates payload-independent artefacts that confound the readout.
Reformulate for in vivo
Bio-Courier® TL
Cells stay viable, so what the assay measures is the payload.
No reformulation for in vivo
Same carrierfrom cells to animals
The same nanoparticle, now as a drug carrier
Low toxicity
In animals as well as in cell culture
Extrahepatic delivery
Delivery beyond the liver
Multiple administration routes
Flexibility in study design
Ease of use
A fully formed empty nanoparticle, loaded in under an hour. Formulations stay stable without an ultra-cold chain.
With the carrier held constant, in vitro data are a better indicator of in vivo performance.
Why Bio-Courier® TL works both in vitro and in vivo.
Bio-Courier® TL stabilises lipids with a silicon matrix and requires less cationic charge than other reagents. This results in effective transfection with less toxicity in both cell culture and in animals.
- LIPIDS Promote endosomal escape and cell entry
- SILICON MATRIX Highly ordered porous structure with exceptional mechanical integrity
- RNA PAYLOAD Electrostatically bound and stabilised RNA enables efficient transfection
Cationic transfection reagents: the culture dish
One high cationic charge does two jobs: it condenses and holds the nucleic acid, and it drives cell entry. Charge scales with payload, and the charge that delivers is the charge that disrupts membranes. Efficacy and toxicity are coupled, which is what keeps these reagents in the dish.
Ionisable lipid nanoparticles: the systemic route
Ionisable lipids bind the payload tightly and release it only once the endosome acidifies. That same tight binding limits release inside cultured cells. The chemistry is tuned for the bloodstream, not the culture plate.
Bio-Courier®: both
A silicon matrix does the structural work: it stabilises the particle and holds the nucleic acid. The cationic lipid is then left with one job — providing the surface that meets the cell — so far less cationic charge is needed for efficient transfection. Less charge means the toxicity that comes with charge does not follow the reagent into the animal.
Bio-Courier® TL: the in vivo advantage
In vivo, Bio-Courier® TL offers numerous advantages over other in vivo reagents and conventional LNPs.
- Unlike conventional LNPs, which are formed around their cargo, Bio-Courier® TL is a finished empty nanoparticle: the payload is loaded afterwards, in under an hour at room temperature, at above 95% encapsulation efficiency.
- The formulations need no ultra-cold chain.
- Multiple administration routes: intravenous, subcutaneous, intraperitoneal, intramuscular, oral, dermal and eye-drop delivery have all been demonstrated.
- Reduced hepatic accumulation, and delivery to other organs and tissues.
- Bio-Courier® TL is well tolerated: more than 1,300 animals have been dosed without adverse reactions.
- In addition to non-GLP in vivo studies, Bio-Courier® TL provides a defined path to IND-enabling studies and the clinic.
One reagent, many payloads.
PAYLOADS
- siRNA
- miRNA
- mRNA
- pDNA
- CRISPR/Cas9 (mRNA + sgRNA)
- CRISPR RNP
WORKFLOW
- Point-of-use loading
- Room-temperature preparation
- No heat step
- No microfluidics
SETTINGS
- In vitro
- Ex vivo
- In vivo
Register for an evaluation kit.
Bio-Courier® TL is preparing for launch. Ahead of general availability we are allocating evaluation kits to a limited number of labs working on nucleic-acid delivery.
Tell us what you work on — it takes about a minute.
- Registered labs are first in line when kits are released.
- Kits ship with the protocol for your application — no method development needed.
- No payment is taken on this form, and there is no obligation.
About SiSaf
Bio-Courier® is developed by SiSaf Ltd, a UK company based in Guildford working on silicon-stabilised delivery of nucleic acids. Bio-Courier® TL is the research-use form of the technology.
Enquiries, partnering and licensing → info@sisaf.com